Recombinant Human POLR3K His Protein

SDS-Page: POLR3K Recombinant Protein [NBP2-51771] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human POLR3K His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-108 of Human POLR3K
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLLFCPG CGNGLIVEEG QRCHRFACNT CPYVHNITRK VTNRKYPKLK EVDDVLGGAA AWENVDSTAE SCPKCEHPRA YFMQLQTRSA DEPMTTFYKC CNAQCGHRWR D

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: POLR3K
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 14.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 50% glycerol, 2 mM DTT, 2 mM EDTA
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human POLR3K His Protein

• C11
• DNA-directed RNA polymerase III subunit K
• DNA-directed RNA polymerase III subunit RPC10
• DNA-directed RNA polymerases III 12.5 kDa polypeptide
• hRPC11
• HsC11p
• polymerase (RNA) III (DNA directed) polypeptide K (12.3 kDa)
• polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
• RNA polymerase III 12.5 kDa subunit
• RNA polymerase III subunit (hRPC11)
• RNA polymerase III subunit C10
• RNA polymerase III subunit C11
• RNA polymerase III subunit CII
• RPC10
• RPC11C11-RNP3
• RPC12.5

Background

POLR3K encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: POLR3K