POLR3A Antibody - Azide and BSA Free

Western Blot: POLR3A Antibody [NBP2-94211] - Analysis of extracts of 293T cells, using POLR3A .Exposure time: 90s.

Product Discontinued

POLR3A Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR3A (NP_008986.2). MVKEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLLYGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKRGLKKKISDKCRKKNICHHCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR3A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 155 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for POLR3A Antibody - Azide and BSA Free

• DNA-directed RNA polymerase III largest subunit
• DNA-directed RNA polymerase III subunit A
• DNA-directed RNA polymerase III subunit RPC1
• EC 2.7.7.6
• hRPC155
• POL3A
• polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• RNA polymerase III 155 kDa subunit
• RNA polymerase III subunit C160
• RNA polymerase III subunit RPC155-D
• RPC1
• RPC155RNA polymerase III subunit C1

Background

RNA polymerase III (Pol III) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3A (polymerase (RNA) III (DNA directed) polypeptide A), also known as RPC1 or RPC155, is a 1,390 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase beta chain family. Existing as the largest and catalytic core component of Pol III, POLR3A functions as a DNA-dependent RNA polymerase that catalyzes the conversion of a nucleoside triphosphate into a diphosphate, thereby transcribing DNA into RNA. The gene encoding POLR3A maps to human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. Defects in some of the genes that map to chromosome 10 are associated with Charcot-Marie Tooth disease, Jackson-Weiss syndrome, Usher syndrome, nonsyndromatic deafness, Wolman's syndrome, Cowden syndrome, multiple endocrine neoplasia type 2 and porphyria.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: POLR3A