POLR2J Antibody (1A10) - Azide and BSA Free

Western Blot: POLR2J Antibody (1A10) [H00005439-M02] - Analysis of POLR2J expression in transfected 293T cell line by POLR2J monoclonal antibody (M02), clone 1A10.Lane 1: POLR2J transfected lysate(13.3 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: POLR2J Antibody (1A10) [H00005439-M02] - Detection limit for recombinant GST tagged POLR2J is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1A10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

POLR2J Antibody (1A10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: POLR2J (AAH24165, 1 a.a. ~ 117 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRVAIKDKQEGIE
• Specificity: POLR2J - polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa (1A10)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR2J
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POLR2J Antibody (1A10) - Azide and BSA Free

• DNA-directed RNA polymerase II subunit J-1
• DNA-directed RNA polymerase II subunit RPB11-a
• hRPB14
• MGC71910
• POLR2J1RNA polymerase II subunit B11-a
• polymerase (RNA) II (DNA directed) polypeptide J (13.3kD)
• polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
• RNA polymerase II 13.3 kDa subunit
• RPB11
• RPB11A
• RPB11m

Background

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2J
• Entrez:
  • 5439(Human)
• Uniprot:
  • AAH24165(Human)