POLR2J2 Antibody (OTI3G4)

Western Blot: POLR2J2 Antibody (3G4) [NBP2-03649] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.

Immunocytochemistry/Immunofluorescence: POLR2J2 Antibody (3G4) [NBP2-03649] Staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2.

Immunohistochemistry-Paraffin: POLR2J2 Antibody (3G4) [NBP2-03649] - Staining of paraffin-embedded Human Kidney tissue using anti-POLR2J2 mouse monoclonal antibody.

Flow Cytometry: POLR2J2 Antibody (3G4) [NBP2-03649] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2J2 antibody, and then analyzed by flow cytometry.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI3G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.72 mg/ml

POLR2J2 Antibody (OTI3G4) Summary

• Immunogen: Full length human recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR2J2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 12.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.72 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for POLR2J2 Antibody (OTI3G4)

• DNA directed RNA polymerase II polypeptide J-related
• DNA-directed RNA polymerase II subunit RPB11-b1
• HRPB11B
• MGC105050
• MGC54043
• polymerase (RNA) II (DNA directed) polypeptide J2
• RNA polymerase II subunit B11-b1
• RPB11
• RPB11b1DNA-directed RNA polymerase II subunit J2

Background

This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2J2
• Entrez:
  • 246721(Human)