POLN Antibody

Product Discontinued

POLN Antibody Summary

• Immunogen: POLN (NP_861524, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag.MENYEALVGFDLCNTPLSSVAQKIMSAMHSGDLVDSKTWGKSTETMEVINKSSVKYSVQLEDRKTQSPEKKDLKSLRSQTSRGSAKLSPQSFSVRLTDQL
• Specificity: POLN - polymerase (DNA directed) nu
• Clonality: Polyclonal
• Host: Mouse
• Gene: POLN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: The quality control of this antibody is limited to Western blot on the immunizing protein. It has also been used for ELISA. Abnova's recommended working dilutions for western analysis are as follows: 1:500 dilution for ascites 1:1000 for purified Ig 1:500

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Unpurified antisera so the specific antibody concentration is unknown. Contains 50% glycerol.
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POLN Antibody

• DNA polymerase N
• DNA polymerase nu
• DNA polymerase POL4P
• EC 2.7.7.7
• POL4P
• polymerase (DNA directed) nu

Background

POLN, also known as DNA polymerase nu, is localized to the nucleus and highly expressed in the heart and testis. Current research on POLN is being performed in relation to several diseases and disorders including Fanconi anemia, pneumonia, and tuberculosis. POLN has also been shown to have interactions with PCNA, FANCA, FANCG, FANCD2, and RAD51 in the DNA replication and DNA repair pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for POLN Antibody (H00353497-A01)(2)

Publications using H00353497-A01

• Frank EG, McDonald JP, Karata K et al. A strategy for the expression of recombinant proteins traditionally hard to purify. Anal Biochem 2012-07-13 [PMID: 22828411]
• Moldovan GL, Madhavan MV, Mirchandani KD et al. DNA polymerase POLN participates in crosslink repair and homologous recombination. Mol Cell Biol 2009-12-07 [PMID: 19995904]

Bioinformatics

• Gene Symbol: POLN
• Entrez:
  • 353497(Human)
• Uniprot:
  • NP_861524(Human)