MSH5 Antibody

Images

 
Immunohistochemistry-Paraffin: MSH5 Antibody [NBP1-20946] - Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: MSH5 Antibody [NBP1-20946] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MSH5 Antibody Summary

Immunogen
Peptide with sequence GDLHCEIRDQET corresponding to internal region according to NP_751897.1, NP_002432.1.
Epitope
internal region
Specificity
This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MSH5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 2.5 - 3.75 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.75 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 38 kDa band in human testis, tonsil and thymus lysates after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.9 kDa band according to the smallest reported isoform NP_002432.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human testis shows nuclear staining in spermatocytes.
Publications
Read Publication using NBP1-20946.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MSH5 Antibody

  • DKFZp434C1615
  • G7
  • hMSH5
  • MGC2939
  • mutS (E. coli) homolog 5
  • mutS homolog 5 (E. coli)
  • mutS protein homolog 5
  • MUTSH5
  • NG23

Background

This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair or meiotic recombination processes. This protein is similar to a Saccharomyces cerevisiae protein that participates in meiotic segregation fidelity and crossing-over. This protein forms heterooligomers with another member of this family, mutS homolog 4. Alternative splicing results in four transcript variants encoding three different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MSH5 Antibody (NBP1-20946)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MSH5
Entrez
Uniprot