PMEL17/SILV Antibody (SPM286) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Eq, Rt(-)Species Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
SPM286
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

PMEL17/SILV Antibody (SPM286) [Janelia Fluor® 549] Summary

Immunogen
Membranes from a human melanoma metastasis
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PMEL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PMEL17/SILV Antibody (SPM286) [Janelia Fluor® 549]

  • D12S53EP1
  • gp100
  • ME20
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for PMEL17/SILV Antibody (NBP2-34755JF549) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34755AF350
Alexa Fluor 405 NBP2-34755AF405
Alexa Fluor 488 NBP2-34755AF488
Alexa Fluor 532 NBP2-34755AF532
Alexa Fluor 594 NBP2-34755AF594
Alexa Fluor 647 NBP2-34755AF647
Alexa Fluor 700 NBP2-34755AF700
Alexa Fluor 750 NBP2-34755AF750
Allophycocyanin NBP2-34755APC
Biotin NBP2-34755B
DyLight 350 NBP2-34755UV
DyLight 405 NBP2-34755V
DyLight 488 NBP2-34755G
DyLight 550 NBP2-34755R
DyLight 594 NBP2-34755DL594
DyLight 650 NBP2-34755C
DyLight 680 NBP2-34755FR
DyLight 755 NBP2-34755IR
FITC NBP2-34755F
HRP NBP2-34755H
Janelia Fluor 549 NBP2-34755JF549
Janelia Fluor 646 NBP2-34755JF646
PE NBP2-34755PE
PerCP NBP2-34755PCP

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