PMEL17/SILV Antibody (SPM142) [DyLight 350]

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Product Details

Summary
Reactivity Hu, Ca(-), Rt(-)Species Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM142
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

PMEL17/SILV Antibody (SPM142) [DyLight 350] Summary

Immunogen
Extract of pigmented melanoma metastases from lymph nodes
Localization
Cytoplasmic
Marker
Melanoma Marker
Specificity
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. It stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PMEL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PMEL17/SILV Antibody (SPM142) [DyLight 350]

  • D12S53EP1
  • gp100
  • ME20
  • ME20-M
  • melanocyte protein mel 17
  • Melanocyte protein Pmel 17
  • Melanocytes lineage-specific antigen GP100
  • Melanoma-associated ME20 antigen
  • melanosomal matrix protein17
  • PMEL17P100
  • premelanosome proteinME20M
  • SI
  • SIL
  • silver (mouse homolog) like
  • silver homolog (mouse)
  • Silver locus protein homolog
  • silver, mouse, homolog of
  • SILVPmel17

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PMEL17/SILV Antibody (NBP2-34739UV) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-34739AF405
Alexa Fluor 488 NBP2-34739AF488
Alexa Fluor 647 NBP2-34739AF647
Alexa Fluor 700 NBP2-34739AF700
Allophycocyanin NBP2-34739APC
Biotin NBP2-34739B
DyLight 350 NBP2-34739UV
DyLight 405 NBP2-34739V
DyLight 488 NBP2-34739G
DyLight 550 NBP2-34739R
DyLight 650 NBP2-34739C
DyLight 680 NBP2-34739FR
DyLight 755 NBP2-34739IR
HRP NBP2-34739H
Janelia Fluor 549 NBP2-34739JF549
Janelia Fluor 646 NBP2-34739JF646
PE NBP2-34739PE
PerCP NBP2-34739PCP

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Bioinformatics Tool for PMEL17/SILV Antibody (NBP2-34739UV)

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Bioinformatics

Gene Symbol PMEL