PMEL17/SILV Antibody (HMB45) - Bioreactor Concentrate

Product Discontinued

PMEL17/SILV Antibody (HMB45) - Bioreactor Concentrate Summary

• Immunogen: Extract of pigmented melanoma metastases from lymph nodes
• Localization: Cytoplasmic
• Marker: Melanoma Marker
• Specificity: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMEL
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5-10ul/million cells
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100-1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Immunoprecipitation 5-10ul/500ug protein lysate
  • Western Blot 1:100-1:200
• Application Notes: Western Blot analysist application cited in literature.
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Culture medium
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for PMEL17/SILV Antibody (HMB45) - Bioreactor Concentrate

• D12S53EP1
• Gp100
• ME20
• ME20M
• ME20-M
• melanocyte protein mel 17
• Melanocyte protein Pmel 17
• Melanocytes lineage-specific antigen GP100
• Melanoma-associated ME20 antigen
• melanosomal matrix protein17
• PMEL
• PMEL17
• PMEL17P100
• premelanosome proteinME20M
• SI
• SIL
• SILV
• silver (mouse homolog) like
• silver homolog (mouse)
• Silver locus protein homolog
• silver, mouse, homolog of
• SILVPmel17

Background

pMel17 is preferentially expressed in melanoma (s) and is not found in normal tissues or carcinomas. It is expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMEL