PMCA1 Antibody

Western Blot: PMCA1 Antibody [NBP1-97754] - (0.03ug/ml) Analysis of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PMCA1 Antibody Summary

• Description: Novus Biologicals Goat PMCA1 Antibody (NBP1-97754) is a polyclonal antibody validated for use in WB and ELISA. Anti-PMCA1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KQDGAIENRNKAKAQD corresponding to internal region according to NP_001673.2.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001001323.1; NP_001673.2).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ATP2B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in HeLa lysates (calculated MW of 135 kDa band according to NP_001673.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PMCA1 Antibody

• ATPase, Ca++ transporting, plasma membrane 1
• EC 3.6.3
• EC 3.6.3.8
• Plasma membrane calcium ATPase isoform 1
• plasma membrane calcium ATPase
• Plasma membrane calcium pump isoform 1
• plasma membrane calcium pump
• plasma membrane calcium-ATPase
• PMCA1kb
• PMCA1plasma membrane calcium-transporting ATPase 1

Background

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PMCA1 Antibody (NBP1-97754)(1)

Publication using NBP1-97754

• Benetti F, Micetic I, Carsughi F et al. Insights into the oligomerization process of the C-terminal domain of humanplasma membrane Ca2+-ATPase. Arch Biochem Biophys 2011-02-15 [PMID: 21126504]

Bioinformatics

• Gene Symbol: ATP2B1
• Entrez:
  • 490(Human)
  • 67972(Mouse)
  • 29598(Rat)
• Uniprot:
  • NP_001001323.1(Human)