Plexin B2 Antibody (537223) [Phycoerythrin]

Human peripheral blood monocytes were stained with Mouse Anti-Human Plexin B2 PE-conjugated Monoclonal Antibody (Catalog # FAB53291P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 537223
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Plexin B2 Antibody (537223) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Plexin B2
  Leu20-Arg1160
  Accession # O15031
• Specificity: Detects human Plexin B2.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLXNB2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin B2 Antibody (537223) [Phycoerythrin]

• MM1
• MM1KIAA0315dJ402G11.3
• Nbla00445
• PLEXB2
• Plexin B2
• plexin-B2
• PLXNB2

Background

Plexin B2 is a 240 kDa member of the B subfamily, plexin family of semaphorin receptors. It is expressed by cerebellar granule cells, neuroepithelium and chondrocytes, and serves as a receptor for Semaphorin 4C. Mature human Plexin B2 is 1819 amino acid (aa) in length. It is a type I transmembrane (TM) glycoprotein that contains an 1178 aa extracellular domain (ECD) plus a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20‑466) and three IPT repeats (aa 803‑1092). The ECD may be cleaved into two subunits, a 170 kDa alpha -chain (aa 20‑1164) and an 80 kDa TM beta ‑chain that remain noncovalently‑linked. Multiple splice variants may exist. One shows an alternate start site at Met1369, while a second shows a deletion of aa 67‑638. Over aa 20‑1160, human Plexin B2 is 82% aa identical to mouse Plexin B2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plexin B2 Antibody (FAB53291P)(1)

Publication using FAB53291P

• Schuster, E;Dashzeveg, NK;Tong, F;Jia, Y;El-Shennawy, L;Zhang, T;Hoffman, AD;Kitata, RB;Kibria, G;Zhang, Y;Squires, JR;Zheng, C;Ramos, E;Taftaf, R;Scholten, D;Almubarak, HF;Adorno-Cruz, V;Sullivan, DP;Reduzzi, C;Minor, AC;Purev-Ochir, W;Spahija, S;Xu, R;Siziopikou, KP;Platanias, LC;Shah, A;Muller, WA;Gradishar, WJ;Cristofanilli, M;Tsai, CF;Shi, T;Liu, H; Computational ranking identifies Plexin-B2 in circulating tumor cell clustering with monocytes in breast cancer metastasis Nature communications 2025-08-16 [PMID: 40818975] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PLXNB2
• Uniprot:
  • O15031()