Plectin Antibody (4C3)

Immunocytochemistry/Immunofluorescence: Plectin Antibody (4C3) [H00005339-M03] - Analysis of monoclonal antibody to PLEC1 on HeLa cell. Antibody concentration 15 ug/ml

ELISA: Plectin Antibody (4C3) [H00005339-M03] - Detection limit for recombinant GST tagged PLEC1 is 0.1 ng/ml as a capture antibody.

Product Discontinued

Plectin Antibody (4C3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PLEC1 (NP_000436, 4384 a.a. ~ 4493 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVPLDEALQRGTVDARTAQKLRDVGAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEA
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLEC
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: It has been used for ELISA and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Plectin Antibody (4C3)

• EBS1
• HD1
• Hemidesmosomal protein 1
• LGMD2Q
• PCNplectin 1, intermediate filament binding protein, 500kD
• PLEC1b
• PLEC1EBSO
• plectin 1, intermediate filament binding protein 500kDa
• plectin
• plectin-1
• PLTNepidermolysis bullosa simplex 1 (Ogna)

Background

Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLEC
• Uniprot:
  • NP_000436(Human)