PLD2 Antibody (846224) [Unconjugated]

Western blot shows lysates of HCT‑116 human colorectal carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PLD2 Monoclonal Antibody (Catalog # MAB10123) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PLD2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 846224
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PLD2 Antibody (846224) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PLD2
  Met1-His117
  Accession # O14939
• Specificity: Detects huma PLD2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLD2 Antibody (846224) [Unconjugated]

• Choline Phosphatase 2
• HPLD2
• PLD1C
• PLD2

Background

Phospholipase D2 (PLD2) is an enzyme encoded by the PLD2 gene. Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. The Phospholipase D (PLD) lipid-signaling enzyme superfamily has long been studied for its roles in cell communication and a wide range of cell biological processes. Potent PLD2 inhibitors are available, like VU 0364739 hydrochloride (Tocris Cat. 4171) and ML 298 hydrochloride (Tocris Cat. 4895). Three different PLD2 isoforms -PLD2A, PLD2B and PLD2C- have been described, ranging from 38 to 105 KDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O14939()