PLC-beta 3 Antibody [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Janelia Fluor 525

PLC-beta 3 Antibody [Janelia Fluor® 525] Summary

• Immunogen: E. coli-derived recombinant human PLC-beta 3
  Lys27-Leu246
  Accession # Q01970
• Specificity: Detects human PLC-beta 3 in Western blots. Does not detect recombinant human (rh) PLC-beta 1, rhPLC-beta 2, or rhPLC-beta 4 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PLCB3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PLC-beta 3 Antibody [Janelia Fluor® 525]

• 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3
• EC 3.1.4.11
• FLJ37084
• Phosphoinositide phospholipase C-beta-3
• phospholipase C, beta 3 (phosphatidylinositol-specific)
• Phospholipase C-beta-3
• PLC beta 3
• PLCB3
• PLCbeta 3
• PLC-beta 3
• PLC-beta-3

Background

Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1, 4, 5-triphosphate and diacylglycerol from phosphatidylinositol 4, 5 bisphosphate. A total of eight mammalian PLC isozymes have been described (PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC gamma1, PLC gamma2, PLC delta1 and PLC delta2) with molecular weights ranging from 85 to 150 kDa. The gamma-type enzymes are unique in that they contain SH2 and SH3 domains. Moreover, the two gamma-type enzymes, but not the beta and delta isozymes, are subject to activation by a number of protein tyrosine kinases which associate with their SH2 domains and induce their activation by phosphoryation. In contrast, activation of PLC beta1, PLC beta2 and PLC beta3 is mediated by the alpha subunits of the Gq class of heterotrimeric G proteins and by certain betagamma G protein subunits. The regulatory mechanisms for PLC delta1 and PLC delta2 are as yet not resolved.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLCB3