PLC-beta 3 Antibody

Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF mem-brane was probed with 1 µg/mL Sheep Anti-Human PLC-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 3 was detected at approx-imately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of DU145 human prostate carcinoma cell line, HEK293 human embryonic kidney cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human PLC-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 3 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PLC-beta 3 Antibody Summary

• Immunogen: E. coli-derived recombinant human PLC-beta 3
  Lys27-Leu246
  Accession # Q01970
• Specificity: Detects human PLC-beta 3 in Western blots. Does not detect recombinant human (rh) PLC-beta 1, rhPLC-beta 2, or rhPLC-beta 4 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PLCB3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLC-beta 3 Antibody

• 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-3
• EC 3.1.4.11
• FLJ37084
• Phosphoinositide phospholipase C-beta-3
• phospholipase C, beta 3 (phosphatidylinositol-specific)
• Phospholipase C-beta-3
• PLC beta 3
• PLCB3
• PLCbeta 3
• PLC-beta 3
• PLC-beta-3

Background

The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-δ, -beta , -gamma , -epsilon , -zeta , and -eta . PLC-beta 3 (Phospholipase C beta-3) is a G-protein dependent phosphodiesterase that catalyzes the generation of inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) from phosphatidylinositol 4,5-bisphosphate (IP2), an essential step in the intracellular transduction of many extracellular signals. The PLC-beta subfamily consists of 4 isozymes, beta 1‑4, which differ in their tissue distribution and their ability to be activated by G proteins. PLC-beta 1 and PLC-beta 3 are more widely expressed, whereas PLC-beta 2 is resticted to hematopoietic cells and PLC-beta 4 is limited to certain neuronal cells and the retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PLC-beta 3 Antibody (AF4716)(2)

Publications using AF4716

• Y Hung, CC Chung, YC Chen, YH Kao, WS Lin, SA Chen, YJ Chen Klotho Modulates Pro-Fibrotic Activities in Human Atrial Fibroblasts through Inhibition of Phospholipase C Signaling and Suppression of Store-Operated Calcium Entry Biomedicines, 2022-07-01;10(7):. 2022-07-01 [PMID: 35884879] (Western Blot, Human)
• Kan W, Adjobo-Hermans M, Burroughs M, Faibis G, Malik S, Tall G, Smrcka A M3 muscarinic receptor interaction with phospholipase C beta3 determines its signaling efficiency. J Biol Chem, 2014-03-04;289(16):11206-18. 2014-03-04 [PMID: 24596086] (Immunoprecipitation, Western Blot, Human)

Bioinformatics

• Gene Symbol: PLCB3
• Uniprot:
  • Q01970()