Plasminogen Kringle 5 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Plasminogen Kringle 5 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Plasminogen Kringle 5
  Cys481-Ala563
  Accession # AAA50168
• Specificity: Detects mouse Plasminogen Kringle 5 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human Angiostatin (Kringle 1‑3) is observed. This antibody is expected to show cross-reactivity with mouse plasminogen.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Plg
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plasminogen Kringle 5 Antibody [Unconjugated]

• HAE4
• Plasminogen Kringle 5

Background

Plasminogen is a coenzyme that has five triple disulfide-linked kringle domains. It is converted to plasmin by plasminogen activators. Internal fragments of plasminogen, including the naturally occurring angiostatin isoforms and Kringle 5, can also be generated by proteolytic cleavage. These internal fragments have been shown to be potent angiogenesis inhibitors (1).

1. Cao, Y. et al. (1997) J. Biol. Chem. 272:22924.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plasminogen Kringle 5 Antibody (AF742)(1)

Publication using AF742

• Doni A, Musso T, Morone D, Bastone A, Zambelli V, Sironi M, Castagnoli C, Cambieri I, Stravalaci M, Pasqualini F, Laface I, Valentino S, Tartari S, Ponzetta A, Maina V, Barbieri S, Tremoli E, Catapano A, Norata G, Bottazzi B, Garlanda C, Mantovani A An acidic microenvironment sets the humoral pattern recognition molecule PTX3 in a tissue repair mode. J Exp Med, 2015-05-11;212(6):905-25. 2015-05-11 [PMID: 25964372] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Plg
• Uniprot:
  • AAA50168()