Plakophilin 2 Antibody

Western Blot: Plakophilin 2 Antibody [NBP1-72054] - (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Plakophilin 2 Antibody [NBP1-72054] - 3.75ug/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Plakophilin 2 Antibody Summary

• Description: Novus Biologicals Goat Plakophilin 2 Antibody (NBP1-72054) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Plakophilin 2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RGTAQYSSQKSVEER corresponding to internal region according to NP_004563.2.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_001005242.2; NP_004563.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PKP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.3-1ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3-1.0 ug/ml
• Application Notes: Western blot: Approx 100kDa band observed in Mouse Heart lysates (calculated MW of 88.1kDa according to Mouse NP_080439.1 and 87.8kDa according to Rat NP_001093969.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Prostate.

Reactivity Notes

Expected from sequence similarity: Rat, Canine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Plakophilin 2 Antibody

• ARVD9
• MGC177501
• plakophilin 2
• plakophilin-2

Background

Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 1 mediates increases in desmosomal protein content, desmosome assembly and regulation of cell migration. Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin 4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plakophilin 2 Antibody (NBP1-72054)(1)

Publication using NBP1-72054

• Christensen AH, Benn M, Tybjaerg-Hansen A et al. Missense variants in plakophilin-2 in arrhythmogenic right ventricular cardiomyopathy patients--disease-causing or innocent bystanders? Cardiology 2010-01-01 [PMID: 19955750]

Bioinformatics

• Gene Symbol: PKP2
• Entrez:
  • 5318(Human)
  • 67451(Mouse)
• Uniprot:
  • NP_001005242.2(Human)