PLA2R1 Antibody (CL0485) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: PLA2R1 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of normal human kidney shows very weak membranous positivity in cells in glomeruli.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0485
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PLA2R1 Antibody (CL0485) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PLA2R1 Antibody (CL0485) - Azide and BSA Free (NBP2-52934) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q13018, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLA2R1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for PLA2R1 Antibody (CL0485) - Azide and BSA Free

• 180 kDa secretory phospholipase A2 receptor
• CLEC13C
• CLEC13CC-type lectin domain family 13 member C
• phospholipase A2 receptor 1, 180kDa
• PLA2G1R
• PLA2IR
• PLA2R1
• PLA2-RM-type receptor
• PLA2Rphospholipase A2 receptor 1, 180kD
• secretory phospholipase A2 receptor

Background

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLA2R1