PKA R2 Antibody (Hs-36)

Product Discontinued

PKA R2 Antibody (Hs-36) Summary

• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Specificity: The antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), an intra-acrosomal protein.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKAR2A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Western Blot 1:1000

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris buffered saline (TBS), pH 8.0
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PKA R2 Antibody (Hs-36)

• protein kinase, cAMP-dependent, regulatory, type II, alpha
• RII-alpha subunit

Background

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAR2A