PIWIL4 Antibody (626001)

PIWIL4 was detected in immersion fixed BG01V human embryonic stem cells using Human PIWIL4 Monoclonal Antibody (Catalog # MAB6316) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 626001
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

PIWIL4 Antibody (626001) Summary

• Immunogen: E. coli-derived recombinant human PIWIL4
  Ser102-Gln237
  Accession # Q7Z3Z4
• Specificity: Detects human PIWIL4 in direct ELISAs. In this format, no cross-reactivity with recombinant human (rh) PIWIL2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PIWIL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIWIL4 Antibody (626001)

• DKFZp686P01248
• FLJ36156
• HIWI2
• HIWI2MIWI2
• MIWI2
• PIWI
• PIWIL4
• piwi-like 4 (Drosophila)
• piwi-like protein 4

Background

PIWI-like protein 4 (PIWIL4) is a 97 kDa member of the argonaute family of proteins and the PIWI subfamily. Human PIWIL4 is 852 amino acids (aa) in length and contains one PAZ domain (aa 268-384) and one PIWI domain (aa 546-838). There are three isoforms for human PIWIL4. Isoform 1 is the standard protein. Isoform 2 has a two aa substitution for aa 1-29 in isoform 1 and a deletion of aa 523-852. Isoform 3 has a deletion of aa 1-69 and a deletion of aa 523-852. Human PIWIL4 shares 77% aa sequence identity with mouse and rat PIWIL4. PIWIL4 plays a central role in spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIWIL4
• Uniprot:
  • Q7Z3Z4()