PIWIL1/HIWI Antibody [Alexa Fluor® 488]

• Reactivity: Hu, Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

PIWIL1/HIWI Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PIWIL1/HIWI
  Arg82-Thr290
  Accession # Q96J94
• Specificity: Detects human PIWIL1/HIWI in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PIWIL1/HIWI Antibody [Alexa Fluor® 488]

• HIWI
• HIWIpiwi homolog
• MIWI
• piwi (Drosophila)-like 1
• PIWI
• PIWIL1
• piwi-like 1 (Drosophila)
• piwi-like protein 1

Background

PIWI (P-element-induced wimpy testis; also HIWI and PIWIL1) is a 92-98 kDa member of the Piwi subfamily, Argonaute family of proteins. It is expressed in germline cells, particularly spermatocytes and spermatids, and also appears in CD34+ hematopoietic stem cells plus select tumor types. PIWI binds to a series of 28‑34 nucleic acid long noncoding RNAs that participate in gametogenesis. It does so under the influence of Arg methyltransferases, and in conjunction with Tudor‑containing proteins. Human PIWI/PIWIL1 is 861 amino acids (aa) in length. It contains a methylation target region in the N-terminus, followed by an RNA-binding PAZ domain (aa 277-391) and an RNA-cleaving PIWI domain (aa 555-847). There are two isoform variants. One is widely expressed and shows a three aa substitution for aa 1‑89. The second contains a six aa substitution for aa 824-861. Over aa 82-290, human PIWI (HIWI) shares 95% aa sequence identity with mouse PIWI (MIWI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.