PIP5K1C Knockout HeLa Cell Lysate

Product Discontinued

PIP5K1C Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,5 bp deletion in exon 4 and 1 bp insertion in exon 4
• Gene: PIP5K1C

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for PIP5K1C Knockout HeLa Cell Lysate

• EC 2.7.1
• EC 2.7.1.68
• KIAA0589
• KIAA0589diphosphoinositide kinase
• LCCS3Human homolog of mouse phosphatidylinositol-4-phosphate 5-kinase I-gamma
• Phosphatidylinositol-4-phosphate 5-kinase type I gamma
• phosphatidylinositol-4-phosphate 5-kinase type-1 gamma
• phosphatidylinositol-4-phosphate 5-kinase, type I, gamma
• PIP5K1C
• PIP5K1gamma
• PIP5K1-gamma
• PIP5Kgamma
• PIP5K-GAMMA
• PIP5KIgamma
• PIPKIg_v4
• PtdIns(4)P-5-kinase 1 gamma
• type I PIP kinase

Background

This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Sep 2010]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PIP5K1C