PIM3 Antibody

Western Blot: PIM3 Antibody [NBP1-50553] - Analysis of anti-PIM3 antibody with mouse kidney tissue lysate.

Immunohistochemistry-Paraffin: PIM3 Antibody [NBP1-50553] - Analysis of anti-PIM3 antibody with human spleen at concentration 10 ug/ml.

Product Discontinued

PIM3 Antibody Summary

• Immunogen: KLH-conjugated synthetic peptide between aa 259-288 from C-terminus region of human PIM3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PIM3
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1:1000
• Application Notes: IHC: NBP1-50553 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Please note that cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse samples at this time.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.25 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for PIM3 Antibody

• EC 2.7.11.1
• pim-3 oncogene
• PIM3
• pim-3
• serine/threonine kinase Pim-3
• serine/threonine-protein kinase pim-3

Background

PIM3 belongs to a family of protooncogenes that encode serine/threonine protein kinases (Mikkers et al., 2004 [PubMed 15199164]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PIM3
• Entrez:
  • 415116(Human)
• Uniprot:
  • Q86V86(Human)