PILR-beta Antibody

Immunohistochemistry-Paraffin: PILR-beta Antibody [NBP1-52373] - Analysis of anti-PILRB antibody with human kidney at concentration 3 ug/ml.

Immunohistochemistry-Paraffin: PILR-beta Antibody [NBP1-52373] - Analysis of anti-PILRB antibody with human pancreas at concentration 3 ug/ml.

Immunohistochemistry-Paraffin: PILR-beta Antibody [NBP1-52373] - Analysis of anti-PILRB antibody with human testis at concentration 3 ug/ml.

Product Discontinued

PILR-beta Antibody Summary

• Immunogen: Synthetic peptide C-RVTESKGHSESWH from an internal region of human PILRB (NP_038468.3; NP_839956.1).
• Specificity: Human PILRB. Both variants represent identical proteins (NP_038468.3; NP_839956.1). This antibody is expected to recognize isoform a only (NP_038468.3; NP_839956.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PILRB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA
• Application Notes: IHC: NBP1-52373 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PILR-beta Antibody

• activating receptor PILRbeta
• Activating receptor PILR-beta
• Cell surface receptor FDFACT
• cell surface receptor FDFACT1
• cell surface receptor FDFACT2
• FDFACT
• FDFACT1
• FDFACT2
• paired immunoglobin-like receptor beta
• paired immunoglobin-like type 2 receptor beta
• paired immunoglobulin-like receptor beta
• paired immunoglobulin-like type 2 receptor beta
• PILRB
• PILRbeta
• PILR-beta

Background

Cell signaling pathways rely on a dynamic interaction between activating and inhibiting processes. SHP-1-mediated dephosphorylation of protein tyrosine residues is central to the regulation of several cell signaling pathways. Two types of inhibitory receptor superfamily members are immunoreceptor tyrosine-based inhibitory motif (ITIM)-bearing receptors and their non-ITIM-bearing, activating counterparts. Control of cell signaling via SHP-1 is thought to occur through a balance between PILRalpha-mediated inhibition and PILRbeta-mediated activation. These paired immunoglobulin-like receptor genes are located in a tandem head-to-tail orientation on chromosome 7. This particular gene encodes the non-ITIM-bearing member of the receptor pair, which has a truncated cytoplasmic tail relative to its ITIM-bearing partner and functions in the activating role. Alternative splicing has been observed at this locus and three variants, encoding two distinct isoforms, are described. Additional transcript variants have been identified but their full-length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PILRB
• Entrez:
  • 29990(Human)
• Uniprot:
  • Q9UKJ0()