Phosphodiesterase 4D Antibody

Western Blot: Phosphodiesterase 4D Antibody [NB100-57076] - (0.01ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

Phosphodiesterase 4D Antibody Summary

• Immunogen: Peptide with sequence HVNNFPFRRHS-C corresponding to N-Terminus according to NP_006194.2.
• Epitope: HVNNFPFRRHS-C
• Specificity: This antibody is expected to recognize isoform 3 (NP_006194.2) only.
• Predicted Species: Human (100%), Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDE4D
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in rat brain lysates (calculated MW of 76.5 kDa band according to human NP_006194.2).
• Control:

  Phosphodiesterase 4D Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Phosphodiesterase 4D Antibody

• cAMP-specific (dunce (Drosophila)-homologphosphodiesterase E3)
• cAMP-specific 3'-5'-cyclic phosphodiesterase 4D
• cAMP-specific phosphodiesterase PDE4D6
• DKFZp686M11213
• DPDE3
• Drosophila)
• dunce-like phosphodiesterase E3
• EC 3.1.4
• FLJ97311
• HSPDE4D
• PDE43
• PDE4D
• Phosphodiesterase 4D
• phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog
• phosphodiesterase 4D, cAMP-specific

Background

Cyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprise of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein protein interactions with cytoskeletal scaffolding proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Phosphodiesterase 4D Antibody (NB100-57076)(1)

Publication using NB100-57076

• Houslay, M.D, Baillie et al. cAMP-Specific phosphodiesterase-4 enzymes in the cardiovascular system: amolecular toolbox for generating compartmentalized cAMP signaling. Circ Res 2007-04-13 [PMID: 17431197]

Bioinformatics

• Gene Symbol: PDE4D
• Entrez:
  • 5144(Human)
  • 24627(Rat)
  • 24627(Rat)
• Uniprot:
  • AAA97892.1(Human)