PHLPP2 Antibody

Western Blot: PHLPP2 Antibody [NB100-61668] - Staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PHLPP2 Antibody Summary

• Immunogen: Peptide with sequence C-PHEEDRTEPPEEFD corresponding to C-Terminus according to NP_055835.2.
• Epitope: PHEEDRTEPPEEFD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PHLPP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx. 140 kDa band observed in human brain (amygdala, hippocampus, substantia nigra) lysates (calculated MW of 134 kDa band according to NP_055835.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PHLPP2 Antibody

• EC 3.1.3.16
• KIAA0931PH domain leucine-rich repeat-containing protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase 2
• PH domain and leucine rich repeat protein phosphatase-like
• PH domain leucine-rich repeat-containing protein phosphatase-like
• PHLPPLPHLPP-like

Background

PHLPP2 contains 21 LRR (leucine rich) repeats, 1 PH domain and 1 PP2C like domain. It is a protein phosphatase that specifically mediates dephosphorylation of AKT1 Ser 473, a protein that regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro and antiapoptotic genes. Dephosphorylation of Ser 473 of AKT1 triggers apoptosis and decrease cell proliferation. PHLPP2 also controls the phosphorylation of AKT3. It binds 2 manganese ions per subunit. There are 3 named isoforms produced by alternative splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHLPP2 Antibody (NB100-61668)(1)

Publication using NB100-61668

• Gao T, Furnari F, Newton AC. PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol Cell 2005-04-01 [PMID: 15808505]

Bioinformatics

• Gene Symbol: PHLPP2
• Entrez:
  • 23035(Human)
• Uniprot:
  • NP_055835.2(Human)