PHGDH Overexpression Lysate

Images

 
Western Blot: PHGDH Overexpression Lysate (Adult Normal) [NBL1-14363] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHGDH.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PHGDH Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203949 and based on accession number NM_006623
Epitope
C-terminal DDK tag
Specificity
Homo sapiens phosphoglycerate dehydrogenase (PHGDH), mRNA.
Gene
PHGDH

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PHGDH Overexpression Lysate

  • 3,3-phosphoglycerate dehydrogenase
  • 3PGDH
  • 3-PGDH
  • D-3-phosphoglycerate dehydrogenase
  • EC 1.1.1
  • EC 1.1.1.95
  • MGC3017
  • PDG
  • PDGH3
  • PGAD
  • PGD
  • PGDH
  • PHGDH
  • phosphoglycerate dehydrogenase
  • SERA

Background

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Other Available Formats

NBL1-14363

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