Recombinant Human PHF5A His Protein

SDS-Page: PHF5A Protein [NBP2-23325]

Product Discontinued

Recombinant Human PHF5A His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-110 of Human PHF5A
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAKHHPD LIFCRKQAGV AIGRLCEKCD GKCVICDSYV RPCTLVRICD ECNYGSYQGR CVICGGPGVS DAYYCKECTI QEKDRDGCPK IVNLGSSKTD LFYERKKYGF KKR

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PHF5A
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 14.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human PHF5A His Protein

• bK223H9.2
• INI
• MGC1346
• PHD finger protein 5A
• PHD finger-like domain protein 5A
• PHD finger-like domain-containing protein 5A
• PHD-finger 5a
• Rds3
• SAP14b
• SF3b14bSplicing factor 3B-associated 14 kDa protein
• splicing factor 3B associated 14 kDa protein

Background

PHF5A is a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. PHF5A contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. Recombinant human PHF5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PHF5A