PHF5A Overexpression Lysate (Native)

Images

 
Western Blot: PHF5A Overexpression Lysate (Adult Normal) [NBL1-14360] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PHF5A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PHF5A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200284 and based on accession number NM_032758. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens PHD finger protein 5A (PHF5A), mRNA.
Gene
PHF5A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PHF5A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PHF5A Overexpression Lysate (Native)

  • bK223H9.2
  • INI
  • MGC1346
  • PHD finger protein 5A
  • PHD finger-like domain protein 5A
  • PHD finger-like domain-containing protein 5A
  • PHD-finger 5a
  • Rds3
  • SAP14b
  • SF3b14bSplicing factor 3B-associated 14 kDa protein
  • splicing factor 3B associated 14 kDa protein

Background

This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PHF5A