PGA5 Antibody [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

PGA5 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Pepsinogen A5/PGA5
  Met1-Ala388
  Accession # P0DJD7
• Specificity: Detects human Pepsinogen A5/PGA5 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PGA5 Antibody [Alexa Fluor® 350]

• EC 3.4.23
• EC 3.4.23.1
• pepsin A
• pepsinogen 5, group I (pepsinogen A)
• Pepsinogen A5
• PGA3
• PGA4
• PGA5

Background

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens and then converted to active pepsins by the acidic pH of gastric juices (1). PGA-3, PGA-4, and PGA-5 are isozymogens of human Pepsinogen A, which differ in amino acid sequence by 2-4 residues (2). This recombinant human Pepsinogen A corresponds to PGA-4. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin. Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.