![Immunoprecipitation: PFDN2 Antibody [NBP3-00279] - PFDN2 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL NBP3-00279 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00279, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size: 17 kDa](http://images.novusbio.com/fullsize/nbp3-00279_rabbit-polyclonal-pfdn2-antibody-235202511354126.png "Immunoprecipitation: PFDN2 Antibody [NBP3-00279] - PFDN2 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL NBP3-00279 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00279, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size: 17 kDa")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles. |
| Immunogen | Produced in rabbits immunized with purified, recombinant Human PFDN2 (Uniprot#: Q9UHV9; Met1-Ser154) |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PFDN2 |
| Purity | Antigen and protein A Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.2 um filtered solution in PBS |
| Preservative | No Preservative |
| Purity | Antigen and protein A Affinity-purified |
| Publication using NBP3-00279 | Applications | Species |
|---|---|---|
| He Q, Ding Z, Chen T et al. PFDN2 promotes cell cycle progression via the hnRNPD-MYBL2 axis in gastric cancer Frontiers in Oncology 2023-07-18 [PMID: 37538116] (IP, WB, Human) Details: WB Dilution: 1:1000; CO-IP |
IP, WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for PFDN2 Antibody (NBP3-00279)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PFDN2 |
![Western Blot: PFDN2 Antibody [NBP3-00279] - NBP3-00279 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:17 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/nbp3-00279_rabbit-polyclonal-pfdn2-antibody-235202521525045.png "Western Blot: PFDN2 Antibody [NBP3-00279] - NBP3-00279 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:17 kDa (We are unsure as to the identity of these extra bands.)")
![Immunohistochemistry Glycophorin A Antibody (R10) [Unconjugated]](https://images.novusbio.com/images2/Glycophorin_A_MAB1228_Immunohistochemistry_19187.jpg)
![Flow Cytometry Glycophorin A Antibody (R10) [Unconjugated]](https://images.novusbio.com/images2/Glycophorin_A_MAB1228_Flow_Cytometry_19288.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")