Peroxiredoxin 3 Antibody (774406) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 774406
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Peroxiredoxin 3 Antibody (774406) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human Peroxiredoxin 3
  Met1-Gln256
  Accession # P30048
• Specificity: Detects human Peroxiredoxin 3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Peroxiredoxin 1, 2, 4, or recombinant mouse Peroxiredoxin 3 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Peroxiredoxin 3 Antibody (774406) [Alexa Fluor® 532]

• Antioxidant protein 1MGC104387
• AOP-1
• AOP-1MGC24293
• AOP1PRO1748
• EC 1.11.1.15
• HBC189
• MER5
• Peroxiredoxin 3
• Peroxiredoxin III
• Peroxiredoxin-3
• PRDX3
• Protein MER5 homolog
• Prx-III
• SP-22
• thioredoxin-dependent peroxide reductase, mitochondrial

Background

Peroxiredoxin 3 (Prx-3/III; also AOP-1, MER5 and thioredoxin-dependent peroxidase reductase) is a ubiquitous, 22-28 kDa mitochondrial antioxidant enzyme that belongs to the 2-Cys class of the TSA/ahpC family of peroxiredoxins. Prx-3 is known to act as either a homodimer, or a decamer, and scavenge reactive oxygen species generated by oxidative stress. The mouse Prx-3 precursor molecule is 257 amino acids (aa) in length. It contains a cleavable N-terminal 63 aa mitochondrial targeting sequence, plus a 194 aa mature enzyme that shows a thioredoxin domain between aa 64-222. There are two catalytic cysteines, one at Cys109 and another at Cys230 of the precursor. Prx-3 undergoes a phosphorylation at Thr147 that reduces its activity. One potential splice form shows a deletion of Arg149, Lys150 and Arg185. Full-length mouse Prx-3 is 86% and 95% aa identical to human and rat Prx-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.