Pepsinogen C/PGC/Progastricsin Antibody

Product Discontinued

Pepsinogen C/PGC/Progastricsin Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 280-330 of Human Pepsin C.
• Specificity: Progastricsin (Y393/439) pAb detects endogenous levels of Abl1/2 protein only when phosphorylated at Y393/439.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PGC
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analyzes of Pepsin C (F306) pAb in extracts from Jurkat cells.
  Immunohistochemistry (IHC) analyzes of Pepsin C (F306) pAb in paraffin-embedded human lung carcinoma tissue.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human and Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pepsinogen C/PGC/Progastricsin Antibody

• EC 3.4.23
• EC 3.4.23.3
• FLJ99563
• Gastricsin
• PEPC
• pepsin C
• Pepsinogen C
• pepsinogen group II
• PGC
• PGII
• preprogastricsin
• progastricsin (pepsinogen C)

Background

The Abl oncogene was initially identified as the viral transforming gene of Abelson murine leukemia virus (A-MuLV). The major translational product of c-Abl has been identified as a protein with tyrosine kinase activity and an SH2 domain. The Abl oncogene is implicated in several human leukemias including 90-95% of chronic myelocytic leukemia (CML), 20-25% of adult acute lymphoblastic leukemia (ALL) and 2-5% of pediatric ALL. In these leukemias the c-Abl proto-oncogene undergoes a (9;22) chromosomal translocation producing the Philadelphia (Ph1) chromosome. The molecular consequence of this translocation is the generation of a chimeric Bcr/c-Abl mRNA encoding activated Abl protein-tyrosine kinase. The Bcr gene has been shown to encode a GTPase-activating protein (GAP) specific for the Ras-related GTP-binding protein, p21rac.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGC