Recombinant Human PDIA6 His Protein

SDS-Page: Recombinant Human PDIA6 Protein [NBP2-52142] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human PDIA6 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 20-440 of Human PDIA6
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLYSSSDDVI ELTPSNFNRE VIQSDSLWLV EFYAPWCGHC QRLTPEWKKA ATALKDVVKV GAVDADKHHS LGGQYGVQGF PTIKIFGSNK NRPEDYQGGR TGEAIVDAAL SALRQLVKDR LGGRSGGYSS GKQGRSDSSS KKDVIELTDD SFDKNVLDSE DVWMVEFYAP WCGHCKNLEP EWAAAASEVK EQTKGKVKLA AVDATVNQVL ASRYGIRGFP TIKIFQKGES PVDYDGGRTR SDIVSRALDL FSDNAPPPEL LEIINEDIAK RTCEEHQLCV VAVLPHILDT GAAGRNSYLE VLLKLADKYK KKMWGWLWTE AGAQSELETA LGIGGFGYPA MAAINARKMK FALLKGSFSE QGINEFLREL SFGRGSTAPV GGGAFPTIVE REPWDGRDGE LPVEDDIDLS DVELDDLGKD EL

• Details of Functionality: Specific activity > 20 A650/cm/min/mg. Enzymatic activity was confirmed by measuring the aggregation of insulin in the presence of DTT.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDIA6
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 48.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 50 mM NaCl, 2 mM DTT, and 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human PDIA6 His Protein

• EC 5.3.4.1
• Endoplasmic reticulum protein 5
• ERp5
• ERP5ER protein 5
• P5TXNDC7
• protein disulfide isomerase family A, member 6
• Protein disulfide isomerase P5
• protein disulfide isomerase-associated 6
• protein disulfide isomerase-related protein
• protein disulfide-isomerase A6
• thioredoxin domain containing 7 (protein disulfide isomerase)
• Thioredoxin domain-containing protein 7

Background

Protein disulfide isomerase (PDIA6) ia an enzyme belonging to the protein disulfide isomerase family. Structurally, it contains 2 thioredoxin domains and its function is to catalyze the rearrangement of -S-S- bonds in proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PDIA6