Human PDIA6 ELISA Kit (Colorimetric)

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ELISA: Human PDIA6 ELISA Kit (Colorimetric) [NBP2-81230] - Samples were spiked with high concentrations of Human PDIA6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human PDIA6 ELISA Kit (Colorimetric) [NBP2-81230] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

Human PDIA6 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human PDIA6 in samples. No significant cross-reactivity or interference between Human PDIA6 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.62%
Intra-Assay
CV% < 5.68%
Spike Recovery
87-105%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
PDIA6

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human PDIA6 ELISA Kit (Colorimetric)

  • EC 5.3.4.1
  • Endoplasmic reticulum protein 5
  • ERp5
  • ERP5ER protein 5
  • P5TXNDC7
  • protein disulfide isomerase family A, member 6
  • Protein disulfide isomerase P5
  • protein disulfide isomerase-associated 6
  • protein disulfide isomerase-related protein
  • protein disulfide-isomerase A6
  • thioredoxin domain containing 7 (protein disulfide isomerase)
  • Thioredoxin domain-containing protein 7

Background

Protein disulfide isomerase (PDIA6) ia an enzyme belonging to the protein disulfide isomerase family. Structurally, it contains 2 thioredoxin domains and its function is to catalyze the rearrangement of -S-S- bonds in proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for PDIA6 ELISA Kit (NBP2-81230). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol PDIA6