PDE3B Antibody (SMCP3B) - BSA Free

Western Blot: PDE3B Antibody (SMCP3B) [NBP1-43333] - Lysates prepared from mouse heart (lane 1) or lymph node cells (lane 2) were run under reducing conditions on a 3-8% Tris-Acetate gel. The membrane was probed with 2 ug/mL of Anti-Mouse PDE3B Purified, followed by Anti-Rat IgG HRP.

• Reactivity: Mu
• Applications: WB
• Clone: SMCP3B
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

PDE3B Antibody (SMCP3B) - BSA Free Summary

• Immunogen: His-tagged fusion protein corresponding to amino acid residues 95-565 from human SMRT.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: PDE3B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: This SMCP3B antibody has been tested by immunoblotting of mouse lymph node cells and mouse heart under reducing conditions. It can be used at 1-5 ug/mL.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for PDE3B Antibody (SMCP3B) - BSA Free

• CGIP1
• CGI-PDE B
• CGIPDE1
• cGMP-inhibited 3'-5'-cyclic phosphodiesterase B
• Cyclic GMP-inhibited phosphodiesterase B
• cyclic nucleotide phosphodiesterase
• EC 3.1.4
• EC 3.1.4.17
• HcGIP1
• PDE3B
• phosphodiesterase 3B, cGMP-inhibited

Background

Cyclic nucleotide phosphodiesterases (PDE) catalyze the hydrolysis of cAMP and cGMP thereby regulating the effects of these second messengers. There are several families of PDE which share common structural organization. The PDE3 family has two members, 3A and 3B that are products of two separate genes. PDE3B (~130 kDa) is expressed predominantly in adipose tissue and lymphocytes, while PDE3A (~120 kDa) is expressed predominantly in platelets and heart tissue. The monoclonal antibody SMCP3B reacts with mouse PDE3B. The SMCP3B antibody was generated against amino acids 643-1100 in the C-terminus and does not cross-react with mouse PDE3A, as determined by western blot analysis of lysates of mouse heart tissue. The SMCP3B antibody does not cross-react with human PDE3B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDE3B Antibody (NBP1-43333)(1)

Publication using NBP1-43333

• Anandagoda N, Willis JC, Hertweck A et al. microRNA-142-mediated repression of phosphodiesterase 3B critically regulates peripheral immune tolerance J. Clin. Invest. 2019-03-01 [PMID: 30741720] (WB, Mouse)

Bioinformatics

• Gene Symbol: PDE3B