PDE1A Antibody

Western Blot: PDE1A Antibody [NBP1-51996] - 3.75ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: PDE1A Antibody [NBP1-51996] - Western blot analysis of PDE1A in Human Cerebellum lysate (35ug protein in RIPA buffer) using NBP1-51996 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PDE1A Antibody Summary

• Immunogen: Peptide with sequence C-DLHKNSEDLVNAE corresponding to C-Terminus according to NP_005010.2.
• Epitope: C-DLHKNSEDLVNAE
• Specificity: This antibody is expected to recognize isoform 1 (NP_005010.2) only.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDE1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-3ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1:100-1:2000
• Application Notes: Western blot: Approx 55kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 62.3kDa according to NP_005010.2). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Kidney.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PDE1A Antibody

• 3'-5' cyclic nucleotide phosphodiesterase
• 61 kDa Cam-PDE
• calcium/calmodulin-dependent 3'-5'-cyclic nucleotide phosphodiesterase 1A
• calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase
• calmodulin-dependent phosphodiesterase
• Cam-PDE 1A
• EC 3.1.4
• EC 3.1.4.17
• HCAM1
• hCam-1
• HSPDE1A
• MGC26303
• PDE1A
• phosphodiesterase 1A, calmodulin-dependent
• phosphodiesterase-1A

Background

PDE1A, a variant of PDE1, is a Ca2+-calmodulin-stimulated phosphodieterase (PDE) preferentially hydrolyzing cGMP. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, B, and C). PDE1A and PDE1B have higher affinity for cGMP than for cAMP, whereas PDE1C has high affinity for both cAMP and cGMP. The PDE1A gene family includes various proteins that differ from each other at amino and carboxy termini of the protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDE1A Antibody (NBP1-51996)(1)

Publication using NBP1-51996

• Cabanero M, Laje G, Detera-Wadleigh S, McMahon FJ. Association study of phosphodiesterase genes in the Sequenced Treatment Alternatives to Relieve Depression sample. Pharmacogenet Genomics 2009-03-01 [PMID: 19214142]

Bioinformatics

• Gene Symbol: PDE1A
• Entrez:
  • 5136(Human)
  • 18573(Mouse)
  • 81529(Rat)
• Uniprot:
  • NP_005010.2(Human)