PCPE-1/PCOLCE Overexpression Lysate (Native)

Images

 
Western Blot: PCOLCE Overexpression Lysate (Adult Normal) [NBL1-14186] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PCOLCE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PCPE-1/PCOLCE Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200515 and based on accession number NM_002593. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens procollagen C-endopeptidase enhancer (PCOLCE), mRNA.
Gene
PCOLCE

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
47.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PCPE-1/PCOLCE

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PCPE-1/PCOLCE Overexpression Lysate (Native)

  • PCOLCE
  • PCPE
  • PCPE1
  • PCPE-1
  • PCPE1Type I procollagen COOH-terminal proteinase enhancer
  • procollagen C-endopeptidase enhancer 1
  • procollagen C-endopeptidase enhancer
  • Procollagen COOH-terminal proteinase enhancer 1
  • Procollagen C-proteinase enhancer 1
  • procollagen, type 1, COOH-terminal proteinase enhancer
  • Type 1 procollagen C-proteinase enhancer protein

Background

Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PCPE-1/PCOLCE Lysate (NBL1-14186) (0)

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Video Protocols

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Bioinformatics

Gene Symbol PCOLCE
Entrez