PBK Overexpression Lysate

Images

 
Western Blot: PBK Overexpression Lysate (Adult Normal) [NBL1-14135] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PBK.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PBK Overexpression Lysate Summary

Description

PBK Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC203295

Accession#: NM_018492

Protein Tag: C-MYC/DDK
Gene
PBK

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
35.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PBK Overexpression Lysate

  • Cancer/testis antigen 84
  • CT84MAPKK-like protein kinase
  • EC 2.7.12.2
  • FLJ14385
  • Nori-3PDZ-binding kinase
  • PDZ binding kinase
  • serine/threonine protein kinase
  • Spermatogenesis-related protein kinase
  • SPKT-LAK cell-originated protein kinase
  • TOPKlymphokine-activated killer T-cell-originated protein kinase

Background

This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PBK Lysate (NBL1-14135) (0)

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Video Protocols

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Bioinformatics

Gene Symbol PBK