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PBK Antibody (2C8)

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Western Blot: PBK Antibody (2C8) [NBP2-37514] - Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.
Western Blot: PBK Antibody (2C8) [NBP2-37514] - Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.
Immunocytochemistry/ Immunofluorescence: PBK Antibody (2C8) [NBP2-37514] - Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled ...read more
Immunohistochemistry: PBK Antibody (2C8) [NBP2-37514] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.
Flow Cytometry: PBK Antibody (2C8) [NBP2-37514] - Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).
Western Blot: PBK Antibody (2C8) [NBP2-37514] - Western blot analysis using PBK mAb against human PBK (AA: 50-230) recombinant protein. (Expected MW is 45.8 kDa)
Immunohistochemistry: PBK Antibody (2C8) [NBP2-37514] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.
ELISA: PBK Antibody (2C8) [NBP2-37514] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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PBK Antibody (2C8) Summary

Immunogen
Purified recombinant fragment of human PBK expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PBK
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PBK Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for PBK Antibody (2C8)

  • Cancer/testis antigen 84
  • CT84MAPKK-like protein kinase
  • EC 2.7.12.2
  • FLJ14385
  • Nori-3PDZ-binding kinase
  • PDZ binding kinase
  • serine/threonine protein kinase
  • Spermatogenesis-related protein kinase
  • SPKT-LAK cell-originated protein kinase
  • TOPKlymphokine-activated killer T-cell-originated protein kinase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: PTCRA Overexpression Lysate (Adult Normal) [NBP2-05680] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTCRA.
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Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Immunohistochemistry-Paraffin: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Human Breast Cancer, using PI3K gamma antibody. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: PI 3-Kinase p110 gamma Antibody [NBP2-15071] - Whole cell extract (30ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PI3 kinase p110 gamma antibody [C3], C-term diluted at 1:1000.
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Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PI 3-Kinase p110 delta Antibody (1D11) [NBP2-46404] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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PBK Antibody (2C8)
NBP2-37514
PBK Antibody (2C8)
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

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Control Lysate(s)

PBK Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

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