PBK Antibody (2C8)

Western Blot: PBK Antibody (2C8) [NBP2-37514] - Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.

Immunocytochemistry/Immunofluorescence: PBK Antibody (2C8) [NBP2-37514] - Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: PBK Antibody (2C8) [NBP2-37514] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.

Flow Cytometry: PBK Antibody (2C8) [NBP2-37514] - Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).

Western Blot: PBK Antibody (2C8) [NBP2-37514] - Western blot analysis using PBK mAb against human PBK (AA: 50-230) recombinant protein. (Expected MW is 45.8 kDa)

Immunohistochemistry: PBK Antibody (2C8) [NBP2-37514] - Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.

ELISA: PBK Antibody (2C8) [NBP2-37514] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

PBK Antibody (2C8) Summary

• Immunogen: Purified recombinant fragment of human PBK expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PBK
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for PBK Antibody (2C8)

• Cancer/testis antigen 84
• CT84MAPKK-like protein kinase
• EC 2.7.12.2
• FLJ14385
• Nori-3PDZ-binding kinase
• PDZ binding kinase
• serine/threonine protein kinase
• Spermatogenesis-related protein kinase
• SPKT-LAK cell-originated protein kinase
• TOPKlymphokine-activated killer T-cell-originated protein kinase

Background

PBK encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PBK