Paxillin Antibody

Images

 
Western Blot: Paxillin Antibody [NBP1-19833] - Aanalysis of Paxillin (L25) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Immunohistochemistry-Paraffin: Paxillin Antibody [NBP1-19833] - Human breast carcinoma tissue.
Western Blot: Paxillin Antibody [NBP1-19833] - extracts from COS-7 cells.
Immunohistochemistry-Paraffin: Paxillin Antibody [NBP1-19833] - Paraffin-embedded human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related Paxillin Primary Antibodies

Order Details


    • Catalog Number
      NBP1-19833
    • Availability
      Product Discontinued

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Paxillin Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids N-terminus of Human Paxillin.
Marker
Focal Adhesion Marker
Clonality
Polyclonal
Host
Rabbit
Gene
PXN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analyzes of Paxillin (L25)pAb in extracts from COS-7 cells.
Immunohistochemistry (IHC) analyzes of Paxillin (L25) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-19833 in the following applications:

Publications
Read Publication using
NBP1-19833 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Paxillin Antibody

  • FLJ16691
  • Paxillin
  • PXN

Background

Paxillin is a focal adhesion phosphoprotein that is localized to the cytoskeleton. Phosphorylation of paxillin has been shown to occur in response to PDGF treatment, v-Src transformation or cross-linking of integrins. FAK (focal adhesion kinase) and PYK2 have been shown to phosphorylate paxillin. FAK phosphorylates paxillin specifically on Tyr 118 in vitro. However, FAK phosphorylation does not seem to be required for the recruitment of paxillin to cell adhesion sites. Paxillin may play a role in signal transduction, regulation of cell morphology and the recruitment of structural and signaling molecules to focal adhesions. It has been shown that the amount of paxillin is reduced in mitotic cells by proteolytic downregulation and that paxillin is alternatively phosphorylated on serine rather than on tyrosine and serine during mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Paxillin Antibody (NBP1-19833)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Filter By Species
Human
(1)
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Review for Paxillin Antibody (NBP1-19833) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-19833:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Paxillin NBP1-19833
Enlarge
reviewed by:
Elizabeth Foxall
IF Human 03/28/2014
View

Summary

ApplicationImmunofluorescence
Sample Testedfixed Human monocytic leukaemia (THP-1) cells
SpeciesHuman
LotG22121
CommentsWe have been getting very good localisation using this antibody with STORM

Blocking

Blocking Detailsblocked in 3% BSA in PBS for 30 mins room temperature

Primary Anitbody

Dilution Ratioanti-paxillin rabbit antibody 1:100 in 3% BSA/PBS for 1 hour at room temperature

Secondary Antibody

Secondary Descriptionhome-made double-conjugated STORM dyes (anti-rabbit conjugated to alexa 647 and cy3)
Secondary Manufacturer Cat#N/A
Secondary Concentration1:100

Details

Detection NotesSTORM using Nikon system
Fixation Detailsfixed in 3.6% PFA at room temperature, washed 3x PBS, permeabilised in 1% triton-X-100 5 mins at room temperature, washed 3x PBS
Wash Description4x wash with PBS for 5 mins per wash

Comments

CommentsWe have been getting very good localisation using this antibody with STORM

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Paxillin Antibody (NBP1-19833) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Paxillin Antibody (NBP1-19833)

Discover related pathways, diseases and genes to Paxillin Antibody (NBP1-19833). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Paxillin Antibody (NBP1-19833)

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Blogs on Paxillin.

CRISPR/Cas9: Keep your friends close, but your viruses closer
"CRISPR", or clustered regularly interspaced short palindromic repeats, is an ancient bacterial mechanism that prevents the invasion of foreign pathogens to a host organism.  Specifically, the CRISPR sequence has been identified as a si...  Read full blog post.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Elizabeth Foxall
03/28/2014
Application: IF
Species: Human

Bioinformatics

Gene Symbol PXN
Entrez
Uniprot