Paxillin Antibody (SY23-02)

Western Blot: Paxillin Antibody (SY23-02) [NBP2-67427] - Analysis of Paxillin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: NIH/3T3 cell lysateLane 2: A549 cell lysate

Immunocytochemistry/Immunofluorescence: Paxillin Antibody (SY23-02) [NBP2-67427] - Staining Paxillin in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Paxillin Antibody (SY23-02) [NBP2-67427] - Analysis of paraffin-embedded mouse testis tissue using anti-Paxillin antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Paxillin Antibody (SY23-02) [NBP2-67427] - Staining Paxillin in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Paxillin Antibody (SY23-02) [NBP2-67427] - Staining Paxillin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Paxillin Antibody (SY23-02) [NBP2-67427] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Paxillin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Paxillin Antibody (SY23-02) [NBP2-67427] - Analysis of paraffin-embedded human kideny tissue using anti-Paxillin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Paxillin Antibody (SY23-02) [NBP2-67427] - Analysis of paraffin-embedded mouse ovary tissue using anti-Paxillin antibody. Counter stained with hematoxylin.

Product Discontinued

Paxillin Antibody (SY23-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Paxillin aa 1-59 / 591. (SwissProt: P49023 Human; SwissProt: Q8VI36 Mouse; SwissProt: Q66H76 Rat)
• Localization: Cytoplasm, Cell junction.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PXN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000
• Application Notes: IP: Use at an assay dependent concentration.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Paxillin Antibody (SY23-02)

• FLJ16691
• Paxillin
• PXN

Background

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PXN