Paxillin Antibody (601327) [Alexa Fluor® 532]

• Applications: WB
• Clone: 601327
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Paxillin Antibody (601327) [Alexa Fluor® 532] Summary

• Immunogen: Phosphopeptide containing the human Paxillin Y118 site
  Accession # P49023
• Specificity: Detects Phospho-Paxillin (Y118) in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Paxillin is a 65 kDa cytoskeletal adaptor protein and member of the Paxillin family. Human Paxillin is 591 amino acids (aa) in length and contains four LIM zinc-binding domains. Alternative splicing produces three isoforms. Human Paxillin shares 94% and 85% aa identity with mouse and rat Paxillin, respectively. Paxillin is found at the interface between actin filaments and the plasma membrane, and it localizes to focal adhesions, where it provides a platform for the integration and coordination of adhesion- and growth factor-related signals. Paxillin phosphorylation at tyrosines 31 and 118 is required for integrin-mediated cytoskeletal reorganization, and may play a role in the disassembly of focal adhesions and stress fibers during cellular transformation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.