Paxillin Antibody (5H11)

Product Discontinued

Paxillin Antibody (5H11) Summary

• Immunogen: BALB/C mice were immunized with purified full-length recombinant human paxillin protein.
• Localization: Cell membrane, cytoplasmic
• Marker: Focal Adhesion Marker
• Specificity: This antibody reacts with a protein of 68 kD. Paxillin is a focal adhesion protein and a substrate for several tyrosine kinase such as src, FAK and p210 BCR/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation. This antibody cross-reacts with rat.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PXN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100

Reactivity Notes

Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Ascites
• Preservative: Sodium Azide
• Purity: Unpurified

Alternate Names for Paxillin Antibody (5H11)

• FLJ16691
• Paxillin
• PXN

Background

Paxillin is a 64 kDa cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PXN