PAQR5 Overexpression Lysate

Western Blot: PAQR5 Overexpression Lysate (Adult Normal) [NBL1-14098] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PAQR5.

• Reactivity: Hu
• Applications: WB

PAQR5 Overexpression Lysate Summary

• Description:

  PAQR5 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC205905
  
  Accession#: NM_017705
  
  Protein Tag: C-MYC/DDK
• Gene: PAQR5

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 37.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PAQR5 Overexpression Lysate

• membrane progestin receptor gamma
• MPRGFLJ20190
• Progestin and adipoQ receptor family member 5
• progestin and adipoQ receptor family member VmPR gamma

Background

The steroid progesterone induces the resumption of maturation in oocytes via a nongenomic pathway through binding to a novel, membrane progestin receptor (mPR). This pathway inhibits adenylyl cyclase and reduces intracellular cAMP, and also activates mitogen-activated protein kinase to effect signal transduction pathways. Three distinct groups, designated alpha, beta, and gamma, comprise this gene family. While all contain 7 transmembrane domains, they show distinct distributions in reproductive, neural, kidney and intestinal tissues. These characteristics separate them from nuclear progestin receptors, and instead imply similarity to G-protein coupled receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PAQR5