Pancreatic Lipase Antibody Pair [HRP]

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Sandwich ELISA: Pancreatic Lipase Antibody Pair [HRP] [NBP2-79417] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP, S-ELISA
Conjugate
HRP
Standard Curve Range
15.63-1000 pg/ml (example only; lot dependent)
Sensitivity
15.63 pg/ml (example only; lot dependent)

Order Details

Pancreatic Lipase Antibody Pair [HRP] Summary

Description
Solid Phase sandwich ELISA for the quantitative determination of Human Pancreatic Lipase.
Specificity
This solid phase sandwich ELISA utilizes an antibody specific for Human Pancreatic Lipase.
Standard Curve Range
15.63-1000 pg/ml (example only; lot dependent)
Sensitivity
15.63 pg/ml (example only; lot dependent)
Assay Type
Sandwich ELISA
Sample Volume
100 ul
Gene
PNLIP

Applications/Dilutions

Dilutions
  • Immunoprecipitation 3-5 ug/mg lysate
  • Sandwich ELISA
  • Western Blot 1:2000-1:10000
Application Notes
1. Capture Antibody: Dilute to a working concentration of 2 ug/ml in PBS before coating.
2. Detection Antibody: Dilute to working concentration of 0.5 ug/ml in detection antibody dilution buffer before use.
3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20C to -80C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/ml is recommended.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Capture Antibody: 1 mg/ml of mouse anti-Human Pancreatic Lipase monoclonal antibody (in PBS, pH 7.4).
  2. Detection Antibody: 0.2 mg/ml of rabbit anti-Human Pancreatic Lipase polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  3. Standard: Each vial contains 80 ng of recombinant Human Pancreatic Lipase.

Alternate Names for Pancreatic Lipase Antibody Pair [HRP]

  • EC 3.1.1.21
  • EC 3.1.1.3
  • pancreatic lipasepancreatic triacylglycerol lipase
  • PL
  • PTL
  • triacylglycerol acylhydrolase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PNLIP