pan Actin Antibody (SPM160) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Rt, RbSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
SPM160
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

pan Actin Antibody (SPM160) [Janelia Fluor® 646] Summary

Immunogen
SDS extract of human myocardium.
Localization
Cytoplasmic
Marker
Muscle Cell Marker
Specificity
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACTA1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for pan Actin Antibody (SPM160) [Janelia Fluor® 646]

  • ACTA
  • actin, alpha 1, skeletal muscle
  • alpha skeletal muscle actin
  • alpha skeletal muscle
  • alpha-actin-1
  • ASMA
  • CFTD
  • CFTDM
  • MPFD
  • NEM1
  • NEM2
  • NEM3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for pan Actin Antibody (NBP2-34399JF646) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34399AF350
Alexa Fluor 405 NBP2-34399AF405
Alexa Fluor 488 NBP2-34399AF488
Alexa Fluor 532 NBP2-34399AF532
Alexa Fluor 594 NBP2-34399AF594
Alexa Fluor 647 NBP2-34399AF647
Alexa Fluor 700 NBP2-34399AF700
Alexa Fluor 750 NBP2-34399AF750
Allophycocyanin NBP2-34399APC
Biotin NBP2-34399B
DyLight 350 NBP2-34399UV
DyLight 405 NBP2-34399V
DyLight 488 NBP2-34399G
DyLight 550 NBP2-34399R
DyLight 594 NBP2-34399DL594
DyLight 650 NBP2-34399C
DyLight 680 NBP2-34399FR
DyLight 755 NBP2-34399IR
FITC NBP2-34399F
HRP NBP2-34399H
Janelia Fluor 549 NBP2-34399JF549
Janelia Fluor 646 NBP2-34399JF646
PE NBP2-34399PE
PerCP NBP2-34399PCP

Additional pan Actin Products

Array NBP2-34399JF646

Bioinformatics Tool for pan Actin Antibody (NBP2-34399JF646)

Discover related pathways, diseases and genes to pan Actin Antibody (NBP2-34399JF646). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for pan Actin Antibody (NBP2-34399JF646)

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Blogs on pan Actin.

"Actin the Fool" about Cytoskeleton Structure
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identifi...  Read full blog post.

Recombinant Monoclonal Antibodies

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Bioinformatics

Gene Symbol ACTA1
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