PAK6 Antibody

Western Blot: PAK6 Antibody [NBP2-19685] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: PAK6 transfected 293T lysates 7. 5% SDS PAGE gel, diluted at 1:5000.

Immunohistochemistry-Paraffin: PAK6 Antibody [NBP2-19685] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.

Immunoprecipitation: PAK6 Antibody [NBP2-19685] - PC-3 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of PAK6 protein by 4 ug PAK6 antibody 7.5 % SDS-PAGE The immunoprecipitated PAK6 protein was detected by PAK6 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Product Discontinued

PAK6 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PAK6. The exact sequence is proprietary.
• Predicted Species: Mouse (97%), Rat (98%), Rhesus Macaque (99%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAK6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PAK6 Antibody

• EC 2.7.11
• p21 protein (Cdc42/Rac)-activated kinase 6
• p21(CDKN1A)-activated kinase 6
• p21-activated kinase 6
• p21-activated protein kinase 6
• PAK-5
• PAK5EC 2.7.11.1
• PAK6
• PAK-6
• serine/threonine-protein kinase PAK 6

Background

This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PAK6
• Uniprot:
  • Q9NQU5()