PACE4/PCSK6 Antibody

Immunohistochemistry-Paraffin: PACE4 Antibody [NBP1-05030] - (3.75ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: PACE4 Antibody [NBP1-05030] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PACE4/PCSK6 Antibody Summary

• Immunogen: Peptide with sequence PDCEPGTYFDSE corresponding to internal region according to NP_612192.1, NP_612195.1, NP_612197.1, NP_612196.1, NP_612198.2, NP_612193.1, NP_612194.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognize reported isoforms a, b, g, h (NP_002561.1; NP_612192.1; NP_612193.1; NP_612194.1 resp.).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PCSK6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1ug/ml
• Application Notes: Western blot: Preliminary experiments gave an approx. 70kDa band in Human, Mouse and Rat Liver lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 105 to 107kDa according to NP_002561.1, NP_612192.1, NP_612193.1 and NP_612194.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Testis and Skeletal Muscle.

Reactivity Notes

Expected from sequence similarity: Mouse and Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PACE4/PCSK6 Antibody

• EC 3.4.21
• EC 3.4.21.61
• EC 3.4.21.75
• PACE4
• PACE4/PCSK6
• PACE4EC 3.4.21.-
• Paired basic amino acid cleaving enzyme 4
• paired basic amino acid cleaving system 4
• PCSK6
• Proprotein Convertase 6
• proprotein convertase subtilisin/kexin type 6
• SPC4
• SPC4subtilisin-like protease
• Subtilisin/kexin-like protease PACE4
• Subtilisin-like proprotein convertase 4

Background

The protein encoded by the PACE4 gene belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. Some of its substrates are - transforming growth factor beta related proteins, proalbumin, and von Willebrand factor. This gene is thought to play a role in tumor progression. Alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PACE4/PCSK6 Antibody (NBP1-05030)(2)

Publications using NBP1-05030

• Su X, Li S, Zhang Y et al. Overexpression of Corin Ameliorates Kidney Fibrosis through Inhibition of Wnt/?-Catenin Signaling in Mice The American journal of pathology 2023-10-10 [PMID: 37827215] (WB, IHC, Mouse)
  
  Details:
  1:100 dilution IHC, 1:500 WB
• Jin W, Wang X, Millar JS et al. Hepatic proprotein convertases modulate HDL metabolism Cell Metab 2007-08-01 [PMID: 17681148]

Bioinformatics

• Gene Symbol: PCSK6
• Entrez:
  • 5046(Human)
  • 18553(Mouse)
  • 25507(Rat)
• Uniprot:
  • NP_002561.1(Human)