p73 [p Tyr99] Antibody

Western Blot: p73 [p Tyr99] Antibody [NB100-56069] - analysis of phosphorylated p73 in (A) recombinant fusion protein containing phosphorylated tyrosine at position 99 and (B) fusion partner without this amino acid, using this antibody. 25 ug/ml.

Product Discontinued

p73 [p Tyr99] Antibody Summary

• Immunogen: A portion of amino acids 75-125, containing a phosphorylated tyrosine residue at position 99, of human p73 was used as the immunogen.
• Modification: p Tyr99
• Specificity: Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described (Sayan et al, 2008). The ability of a given antibody to recognize a p73 fragment will depend on whether or not the antibody epitope is contained in the fragment. As p73 research continues to evolve, researchers are encouraged to refer to the published literature using the p73 antibodies to gain additional information.
• Predicted Species: Mouse (100%), Rat (100%), Chicken (90%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TP73
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Control:

  p73 Overexpression Lysate

Reactivity Notes

Reacts with Human. Predicted to react with Chicken, Chimpanzee, Dog, New World monkey, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for p73 [p Tyr99] Antibody

• p53-related protein
• P73p53-like transcription factor
• TA p73
• TAp73
• tumor protein p73

Background

p73 was identified as a long-lost cousin of tumor suppressor protein, p53. p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP73
• Entrez:
  • 7161(Human)
  • 22062(Mouse)
  • 362675(Rat)
• Uniprot:
  • NP_005418.1(Human)